Composite
Part:BBa_K4759206
Designed by: Jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-07)
T7-RBS4-TF-linker-OleP
We add a TF(solubilization-promoting tag) at the N-terminal of the P450 enzyme
Usage and Biology
We add a solubilization-promoting tag at the N-terminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
Fig1: The application of protein tags
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 591
Illegal AgeI site found at 1428
Illegal AgeI site found at 1526 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 159
Illegal SapI.rc site found at 807
Illegal SapI.rc site found at 920
Illegal SapI.rc site found at 1176
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Categories
Parameters
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